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Tuesday, July 28 • 11:05 - 11:10
Using comparative transcriptomics to dissect the mechanisms of early maturation trait in garden pea (Pisum sativum L.)

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Seed development remains a crucial point in modern molecular plant science. Although mechanisms underlying seed developmental progress have been thoroughly studied, the intraspecies differences in seed developmental rate are poorly dissected in most of the plant species. This problem is especially urgent for crop species, seeds of which are of agricultural and economic value. In this regard comparative transcriptomic and/or proteomic studies of plant accessions belonging to the same species yet differing in their seed development speed offer great opportunities for elucidation of respective mechanisms; however, only few are present to date. In this work we have used whole transcriptome sequencing (RNA-Seq) approach to study the mechanisms underlying early seed maturation feature in garden pea (Pisum sativum L.). An early maturing pea line Sprint-2 was chosen for this study. At the initial point of this work differential expression was estimated between Sprint-2 seeds at 10 and 20 days after pollination, indicating features of late embryogenesis at the former point and seed desiccation at the latter one. Further analysis of differential expression between Sprint-2 and two pea cultivars with intermediate maturation rate at the respective time points proved that Sprint-2 shows developmental retardation at the initial phases of development yet undergoes acceleration between 10 and 20 days after pollination. Further analysis of differentially expressed genes revealed altered gibberellin/abscisic acid ratio to be a putative mechanism underlying Sprint-2 developmental acceleration. We also found several features of early maturation in Sprint-2, which included successive switch between DNA methylation programs, premature activity of amylase genes, enhanced mobile element propagation and retarded storage protein accumulation. Finally, several point mutations absent in other pea cultivars were found in Sprint-2 affecting transcription factors belonging to the LAFL group of maturation regulators. We believe that, coupled with the thorough whole genome association analysis, these results may shed light on the mechanisms of seed maturation speed control and could be further utilized in pea breeding programs.
This work was financially supported by the Russian Science Foundation (grant No 17-16-01100).


Yury V. Malovichko

All-Russia Research Institute for Agricultural Microbiology (ARRIAM), Pushkin, St. Petersburg, Russia, St. Petersburg State University, St. Petersburg, Russia

Tuesday July 28, 2020 11:05 - 11:10 MSK
Zoom Conference https://zoom.us/j/94321101353?pwd=QlJBb09uM0NVVnVyK0FkbTJ3Nkcrdz09