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Tuesday, July 28 • 11:10 - 11:15
Comparative in silico miRNA and their target analysis in Cestrum nocturnum L. and Cestrum diurnum L. in response to stress and flower-anthesis

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The flowers of Cestrum nocturnum L. (CS1) and Cestrum diurnumL. (CS2) bloom at night and at day time, respectively. So, it is interesting to find out the responsible molecular factors especially miRNAs and their underlying mechanism governing the flower-anthesis in these two species. Homology-search based computational analysis was employed  for the identification of miRNAs in CS1 and CS2. A total of twenty six miRNA families (miR5218, miR3513-3p, miR1089, miR815a, miR815b, miR815c, miR1023a-5p, miR2673a, miR2673b, miR1095a, miR1095b, miR172, miR849, miR1063a, miR1063b, miR1063c, miR1063d, miR1063e, miR1063f, miR1063g, miR1063h, miR1070, miR172d, miR1023a-3p, miR5021, miR2919) in CS1 and ten miRNA families (miR5658, miR1533, miR5021, miR5256, miR167a, miR167b, miR167h, miR1436, miR5205a, miR5303) were predicted in CS2. Out of the which, fifteen and nine miRNA families in CS1 and CS2 were identified to regulate 1024 and 1007 corresponding target transcripts. These miRNAs showed significant function in plant circadian rhythm and several stress responses. A biological network of CS1 and CS2 was built based on the role of miRNAs, corresponding gene regulation and their validation. Simulataneously, the gene ontology and pathway analysis indicated that miR815a, miR849, and miR172d had regulatory control over the nocturnal rhythm in CS1 by regulating PIF1, UBP12, EFS and miR5021, miR5658, miR5658, miR1533, miR5205a, miR1436 had regulatory control over the diurnal rhythm of FT, COP, FUS9, GI-FB, PLP6, and SCL13 gene in CS2. MiR2919, miR849, miR2673a, miR5021, miR815a, miR172, miR1089 had a regulatory role over LRR, GRAS, Hsp70 in CS1 and miR5021, miR5303, miR5205a, miR1436 had the regulatory role over LRR, HSP70, MYB48, SOS1, NPC2 in CS2. Some of the corresponding genes were found to be involved in stress responses. The phylogenetic analysis indicates that, the predicted miRNA families display maximum sequence similarity with the reported miRNAs of the same family.

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Tuesday July 28, 2020 11:10 - 11:15 MSK
Zoom Conference https://zoom.us/j/94321101353?pwd=QlJBb09uM0NVVnVyK0FkbTJ3Nkcrdz09