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Tuesday, July 28 • 17:20 - 17:25
Specialized Metabolism Gene Clusters from Red Sea Brine Pool Microbial Metagenomes

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Mining for specialized metabolism gene clusters (SMGCs) is one approach to finding new antibacterial and anticancer natural products, especially from under-explored environments. Microbial metagenomes from Atlantis II Deep, Discovery Deep and Kebrit Deep Red Sea brine pools were shotgun sequenced and 2,751 Red Sea brine SMGCs were detected. The Red Sea brine SMGCs were found to be potentially encoding for natural products pertaining to 28 classes, that were functionally grouped into three main categories, which comprise the following diverse chemistries -in addition to hybrid clusters-: (1) saccharides, fatty acids, aryl polyenes, acyl-homoserine lactones, (2) terpenes, ribosomal peptides, non-ribosomal peptides, polyketides, phosphonates and (3) polyunsaturated fatty acids, ectoine, ladderane and others. We recently reported our findings, and here we will focus on the specific methodology of SMGCs detection in metagenomic samples, and on a particular selected group of natural products, which are the Ribosomally synthesized and post-translationally modified peptides (RiPPs). Although RiPPs constitute only 0.78% of the total Red Sea brine SMGCs, they are technically feasible to test in the lab, and thus it can be selected for prioritization for downstream experimentation. Moreover, several earlier studies have reported RiPPs belonging to similar classes, which exhibited antibacterial and/or anticancer effects. Bacteriocins (17 SMGCs), saccharide-bacteriocin hybrid clusters (3 SMGCs), Microcins (3 SMGCs) and Lanthipeptides (2 SMGCs), constitute the detected Red Sea brine RiPPs. In addition to our earlier reported results, here we will focus more on the methodology and recommendations for optimal mining microbial metagenomes for SMGCs, furthermore, we focus on and prioritize an additional selected group (RiPPs) for recommendation to the experimental work to validate and highlight the importance of the implemented  methodology.

avatar for Laila Ziko

Laila Ziko

Postdoctoral Researcher, Adjunct Assistant Professor, Biology Department, American University in Cairo
I'm a Postdoc interested in different topics that I really like to work on. Natural products from microbes, Metagenomics, antibacterial compounds, anticancer compounds, all are very interesting to me. Reach out for discussion & possible collaboration, look out for my talk on our recent... Read More →

Tuesday July 28, 2020 17:20 - 17:25 MSK
Zoom Conference https://zoom.us/j/94321101353?pwd=QlJBb09uM0NVVnVyK0FkbTJ3Nkcrdz09