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Tuesday, July 28 • 18:30 - 18:35
Preliminary Analysis of Resistome in Mycobacterium abscessus

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Mycobacterium abscessus (Mab), a complex of rapidly growing non-tuberculous mycobacteria, causes human infections that are difficult to treat because of its resistance to multiple antibiotics. The whole genome sequences of 1,581 Mab downloaded from the NCBI FTP site were used to infer phylogenetic relationships and investigate the resistome in silico. A total of 2,975 putative protein sequences of resistance genes from 32 distinct drug classes were detected using Comprehensive Antibiotic Resistance Database (CARD) and ARG-ANNOT databases. The most abundant resistance genes detected were related to beta-lactams (1,962 genes), aminoglycosides (258 genes) and fluoroquinolones (205 genes). These genes encoded (i) many multidrug efflux pumps, such as a homolog of Pseudomonas aeruginosa MexAB-OprM involved in resistance to macrolides, fluoroquinolones, monobactams, carbapenems, cephalosporins, cephamycins, penams, tetracyclines, peptides, aminocoumarin, diaminopyrimidines, sulfonamides, phenicols and penems; (ii) different types of beta-lactamases, for instance, KPC type beta-lactamases that decrease susceptibility to monobactams, carbapenems, cephalosporins, and penams, as well as (iii) various transferases, such as a homolog of mph(B) phosphotransferase from Escherichia coli that decreases susceptibility to macrolides. These findings give insight into the mechanisms of resistance to antibiotics in Mab especially those commonly used to treat Mab infections.

Speakers
SL

Shay Lee Chong

Faculty of Information Science and Technology, Multimedia University, Melaka, Malaysia


Tuesday July 28, 2020 18:30 - 18:35 MSK
Zoom Conference https://zoom.us/j/94321101353?pwd=QlJBb09uM0NVVnVyK0FkbTJ3Nkcrdz09