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Tuesday, July 28 • 18:55 - 19:00
Transcriptome meta-analysis of low phosphate-treated rice roots reveals novel players in phosphate starvation response of crops

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Phosphorus (P) is a major essential macronutrient for plants, and its availability in the soil is scarce and not renewable. Molecular responses to low concentrations of inorganic phosphate (Pi) are better understood in the model plant Arabidopsis thaliana L., whereas several information gaps still exist in crops. Besides, rice has invaluable socioeconomic importance and is a crop model system for studies on Pi starvation responses due to its high tolerance to Pi deprivation. Thus, unraveling the mechanisms related to Pi starvation in rice is extremely important for plant breeding and agronomic productivity and sustainability. In this work, we analyzed transcriptomic data available in the Gene Expression Omnibus (GEO) public repository aiming to identify new components involved in Pi starvation responses in rice roots. Our analysis was based on all datasets available unambiguously specifying the comparison between the transcriptomics of rice (Oryza sativa L. cv. Nipponbare) roots after a Pi-starvation treatment and rice roots grown under a Pi-sufficient condition during the same period. A total of seven datasets from four independent experiments matching these criteria were used. To explore more deeply the candidate genes that are probably the most directly related to Pi starvation responses, we focused only on the top group of genes, that were significantly responsive in at least six datasets for this review. Precisely, 8 and 25 genes were concomitantly up-regulated, and 3 and 34 genes were concomitantly down-regulated in seven and six datasets, respectively, out of all the seven datasets used in this analysis. The selected genes, for both up- and down-regulated groups, displayed different levels of expression, although being generally consistent among the datasets. By analysing the information available in the literature, we were able to group the Pi-responsive genes into two categories: the ones that are well-known players in Pi metabolism or related processes; and the ones that have unknown function or have known functions in processes or pathways other than those related to Pi metabolism. We present the identification of these genes and discuss their known roles with possible relation with Pi metabolism. Moreover, we pinpoint genes probably important for the Pi metabolism but not yet explored, as well as new insights for known genes but not explored in the context of Pi starvation. Orthologs of the promising Pi-responsive genes with unclear function identified in rice roots were also discussed in the context of other crops. Our analysis and discussion offer new alternatives for the development of crops, mainly rice, with improved phosphate acquisition and use efficiency, helping to cope with the global food crisis combined with the panorama of depleting phosphorus resources. This work is part of our review entitled “Phosphate starvation responses in crop roots: from well-known players to novel candidates”, under publication process.

avatar for Yugo Lima-Melo

Yugo Lima-Melo

Postdoctoral researcher, Universidade Federal do Rio Grande do Sul

Tuesday July 28, 2020 18:55 - 19:00 MSK
Zoom Conference https://zoom.us/j/94321101353?pwd=QlJBb09uM0NVVnVyK0FkbTJ3Nkcrdz09